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Examples of Analysis

NHANES HPLC Separation of Carotenoids, Tocopherols, Retinoids
C30 HPLC Separation of Food Carotenoids 
GC Separation of Fatty Acids
HPLC Separation of Ascorbic Acid



NHANES HPLC Method for separation of carotenoids, tocopherols, and retinoids

NHANES HPLC Method for separation of carotenoids, tocopherols, and retinoids

 

Sample Type: Serum or Plasma (Heparinized preferred)

Preferred Amount: >500 µL

Minimum Amount: 100 µL

Limit of Detection:
Retinol <3ng/mL;
Carotenoids <3ng/mL,
Tocopherols <30ng/mL

C30 HPLC Separation of Food Carotenoids

C30 HPLC Separation of Food Carotenoids

Sample Type: Food, Feed, Ingredient, Serum or Plasma (Heparinized preferred)

Preferred Amount: Varies with concentration, >1g

Minimum Amount: Varies with concentration, >100 mg

Limit of Detection:
Carotenoids <15ng/g


GC Chromatogram of Fatty Acids

GC Chromatogram of Fatty Acids

Sample type: Food, Feed, Ingredient, Plasma, Tissue

Preferred amount: Food, Feed, Ingredient, Tissue >10 g
Serum >2 mL

Minimum Amount: Food, Feed, Ingredient, Tissue >1 g
Serum >200 µL

Limit of Detection: Depends on sample concentration; ~0.05 ng of fatty acid


HPLC Chromatogram of Ascorbic Acid

HPLC Chromatogram of Ascorbic Acid

Sample type: Food, Feed, Ingredient, Plasma, Tissue, Urine

Preferred amount: Food, Feed, Ingredient, Tissue >10 g
Serum, Urine >1 mL

Minimum Amount: Food, Feed, Ingredient, Tissue >1 g
Serum, Urine >200 µL

Limit of Detection: Depends on sample concentration; ~10 ng of ascorbic acid

 

 

 

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